chip-it express enzymatic shearing kit (Active Motif)
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chip-it express enzymatic shearing kit
Chip It Express Enzymatic Shearing Kit, supplied by Active Motif, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/chip-it+express+enzymatic+shearing+kit/chip+it+express+enzymatic+shearing+kit/pmc12129438-65-5-15
Average 90 stars, based on 1 article reviews
Chip It Express Enzymatic Shearing Kit, supplied by Active Motif, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/chip-it+express+enzymatic+shearing+kit/chip+it+express+enzymatic+shearing+kit/pmc12129438-65-5-15
Average 90 stars, based on 1 article reviews
chip-it express enzymatic shearing kit - by Bioz Stars,
2026-09
90/100 stars
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Isolation:Article Title: YAP1-mediated regulation of mitochondrial dynamics in IDH1 mutant gliomas. Article Snippet: Jo u rn al o f Ce ll Sc ie n ce sheared enzymatically using Article Title: RORα is critical for mTORC1 activity in T cell-mediated colitis. Article Snippet: Antibodies anti-CD3e eBioscience Cat# 45-0031-82; RRID: AB_1107000 anti-CD4 Biolegend Cat# 100548; RRID: AB_2563054 anti-CD69 BD Biosciences Cat# 557392; RRID: AB_396675 anti-CD127 eBioscience Cat# 48-1271-82; RRID: AB_2016698 anti-Gr1 eBioscience Cat# 48-5931-82; RRID: AB_1548788 anti-CD11b eBioscience Cat# 47-0112-82; RRID: AB_1603193 anti-Siglec F eBioscience Cat# 46-1702-80; RRID: AB_2573724 anti-a4b7 Biolegend Cat# 120608; RRID: AB_10730607 anti-CCR9 eBioscience Cat# 11-1991-82; RRID: AB_657513 anti-CD45 eBioscience Cat# 11-0451-82; RRID: AB_465050 anti-CD45.1 BD Biosciences Cat# 553775; RRID: AB_10926208 anti-CD27 Biolegend Cat# 124216; RRID: AB_10639726 anti-RORgt BD Biosciences Cat# 562607; RRID: AB_11153137 anti-FOXP3 eBioscience Cat# 48-5773-82; RRID: AB_1518812 anti-Bcl-2 Biolegend Cat# 633507; RRID: AB_2043939 anti-IL-17A eBioscience Cat# 48-7177-82; RRID: AB_11149503 anti-IFN-g BD Biosciences Cat# 557998; RRID: AB_396979 anti-TNFa BD Biosciences Cat# 17-7321-82; RRID: AB_469508 anti-mTOR pS2448 eBioscience Cat# 50-9718-42; RRID: AB_2574352 anti-4EBP1 pT36/pT45 BD Biosciences Cat# 560285; RRID: AB_1645498 anti-S6 pS235/pS236 BD Biosciences Cat# 25-9007-42; RRID: AB_2637099 anti-AKT pS473 BD Biosciences Cat# 560397; RRID: AB_1645553 anti-AKT pT308 BD Biosciences Cat# 558275; RRID: AB_2225329 anti-RORa ThermoFisher Cat# PA5-11224; RRID: AB_2285213 Donkey anti-rabbit IgG Biolegend Cat# 406406; RRID: AB_1575135 anti-CD4 (IF) Biolegend Cat# 100443; RRID: AB_2562557 anti-CD127 (IF) BD Biosciences Cat# 552543; RRID: AB_394417 anti-CD69 (IF) BD Biosciences Cat# 557392; RRID: AB_396675 anti-HA CST Cat# 3724 Normal Rabbit IgG CST Cat# 2729 Chemicals, peptides, and recombinant proteins Recombinant Human TGF-b R&D Systems Cat# 240-B-010 Recombinant Mouse IL-6 Peprotech Cat# 216-16 Recombinant Mouse IL-23 R&D Systems Cat# 1887-ML-010 Recombinant Mouse IL-1b Peprotech Cat# 211-11B Critical commercial assays Fixation/Permeabilization Solution Kit BD Biosciences Cat# 554714 FOXP3 / Transcription Factor Staining Buffer Set eBioscience Cat# 00-5523-00 ChIP-IT Express Enzymatic Shearing Kit NEXTflex Active Motif Cat# 53035 (Continued on next page) Cell Reports 36, 109682, September 14, 2021 e1 Article Title: The U1 antisense morpholino oligonucleotide (AMO) disrupts U1 snRNP structure to promote intronic PCPA modification of pre-mRNAs Article Snippet: ChIP-Seq libraries were prepared using the Article Title: Reduced YAP1 and FOLR1 in gliomas predict better response to chemotherapeutics. Article Snippet: Gliomas harbouring mutations in IDH1 (isocitrate dehydrogenase 1) are characterized by greater sensitivity to chemotherapeutics.. These mutants also exhibit diminished levels of transcriptional coactivator YAP1 (yesassociated protein 1).. Enhanced DNA damage in IDH1 mutant cells, as evidenced by γH2AX formation (phosphorylation of histone variant H2A.X) and ATM (serine/threonine kinase; ataxia telangiectasia mutated) phosphorylation, was accompanied by reduced FOLR1 (folate receptor 1) expression. Article Title: Nuclear S100A9 Protein Induces Anti-Inflammatory Gene Expression in Sepsis Article Snippet: Article Title: FAM120A couples SREBP-dependent transcription and splicing of lipogenesis enzymes downstream of mTORC1. Article Snippet: In brief Cho et al. report FAM120A as a transcription co-activator that connects transcription and splicing of de novo lipid synthesis enzymes downstream of mTORC1.. Upon mTORC1 activation, SRPK2 phosphorylates splicing factor SRSF1, inducing its binding with FAM120A.. Through its interaction with SRSF1, the SREBP1-associated FAM120A promotes efficient splicing of lipogenic genes and facilitates lipid metabolism of cancer cells. Article Title: Fungal sensing by dectin-1 directs the non-pathogenic polarization of T H 17 cells through balanced type I IFN responses in human DCs. Article Snippet: Binding of IRF factors to the IFNB promoter was assessed by ChIP assays using the ChIP-IT Express Enzymatic Shearing and Article Title: Inhibiting KDM6A Demethylase Represses Long Non-Coding RNA Hotairm1 Transcription in MDSC During Sepsis. Article Snippet: The cells were transfected and incubated with RPMI-1640 medium for 36 h. Chromatin Immunoprecipitation:Article Title: YAP1-mediated regulation of mitochondrial dynamics in IDH1 mutant gliomas. Article Snippet: Jo u rn al o f Ce ll Sc ie n ce sheared enzymatically using Article Title: RORα is critical for mTORC1 activity in T cell-mediated colitis. Article Snippet: Antibodies anti-CD3e eBioscience Cat# 45-0031-82; RRID: AB_1107000 anti-CD4 Biolegend Cat# 100548; RRID: AB_2563054 anti-CD69 BD Biosciences Cat# 557392; RRID: AB_396675 anti-CD127 eBioscience Cat# 48-1271-82; RRID: AB_2016698 anti-Gr1 eBioscience Cat# 48-5931-82; RRID: AB_1548788 anti-CD11b eBioscience Cat# 47-0112-82; RRID: AB_1603193 anti-Siglec F eBioscience Cat# 46-1702-80; RRID: AB_2573724 anti-a4b7 Biolegend Cat# 120608; RRID: AB_10730607 anti-CCR9 eBioscience Cat# 11-1991-82; RRID: AB_657513 anti-CD45 eBioscience Cat# 11-0451-82; RRID: AB_465050 anti-CD45.1 BD Biosciences Cat# 553775; RRID: AB_10926208 anti-CD27 Biolegend Cat# 124216; RRID: AB_10639726 anti-RORgt BD Biosciences Cat# 562607; RRID: AB_11153137 anti-FOXP3 eBioscience Cat# 48-5773-82; RRID: AB_1518812 anti-Bcl-2 Biolegend Cat# 633507; RRID: AB_2043939 anti-IL-17A eBioscience Cat# 48-7177-82; RRID: AB_11149503 anti-IFN-g BD Biosciences Cat# 557998; RRID: AB_396979 anti-TNFa BD Biosciences Cat# 17-7321-82; RRID: AB_469508 anti-mTOR pS2448 eBioscience Cat# 50-9718-42; RRID: AB_2574352 anti-4EBP1 pT36/pT45 BD Biosciences Cat# 560285; RRID: AB_1645498 anti-S6 pS235/pS236 BD Biosciences Cat# 25-9007-42; RRID: AB_2637099 anti-AKT pS473 BD Biosciences Cat# 560397; RRID: AB_1645553 anti-AKT pT308 BD Biosciences Cat# 558275; RRID: AB_2225329 anti-RORa ThermoFisher Cat# PA5-11224; RRID: AB_2285213 Donkey anti-rabbit IgG Biolegend Cat# 406406; RRID: AB_1575135 anti-CD4 (IF) Biolegend Cat# 100443; RRID: AB_2562557 anti-CD127 (IF) BD Biosciences Cat# 552543; RRID: AB_394417 anti-CD69 (IF) BD Biosciences Cat# 557392; RRID: AB_396675 anti-HA CST Cat# 3724 Normal Rabbit IgG CST Cat# 2729 Chemicals, peptides, and recombinant proteins Recombinant Human TGF-b R&D Systems Cat# 240-B-010 Recombinant Mouse IL-6 Peprotech Cat# 216-16 Recombinant Mouse IL-23 R&D Systems Cat# 1887-ML-010 Recombinant Mouse IL-1b Peprotech Cat# 211-11B Critical commercial assays Fixation/Permeabilization Solution Kit BD Biosciences Cat# 554714 FOXP3 / Transcription Factor Staining Buffer Set eBioscience Cat# 00-5523-00 ChIP-IT Express Enzymatic Shearing Kit NEXTflex Active Motif Cat# 53035 (Continued on next page) Cell Reports 36, 109682, September 14, 2021 e1 Article Title: The U1 antisense morpholino oligonucleotide (AMO) disrupts U1 snRNP structure to promote intronic PCPA modification of pre-mRNAs Article Snippet: ChIP-Seq libraries were prepared using the Article Title: Reduced YAP1 and FOLR1 in gliomas predict better response to chemotherapeutics. Article Snippet: Gliomas harbouring mutations in IDH1 (isocitrate dehydrogenase 1) are characterized by greater sensitivity to chemotherapeutics.. These mutants also exhibit diminished levels of transcriptional coactivator YAP1 (yesassociated protein 1).. Enhanced DNA damage in IDH1 mutant cells, as evidenced by γH2AX formation (phosphorylation of histone variant H2A.X) and ATM (serine/threonine kinase; ataxia telangiectasia mutated) phosphorylation, was accompanied by reduced FOLR1 (folate receptor 1) expression. Article Title: Nuclear S100A9 Protein Induces Anti-Inflammatory Gene Expression in Sepsis Article Snippet: Article Title: FAM120A couples SREBP-dependent transcription and splicing of lipogenesis enzymes downstream of mTORC1. Article Snippet: In brief Cho et al. report FAM120A as a transcription co-activator that connects transcription and splicing of de novo lipid synthesis enzymes downstream of mTORC1.. Upon mTORC1 activation, SRPK2 phosphorylates splicing factor SRSF1, inducing its binding with FAM120A.. Through its interaction with SRSF1, the SREBP1-associated FAM120A promotes efficient splicing of lipogenic genes and facilitates lipid metabolism of cancer cells. Article Title: Fungal sensing by dectin-1 directs the non-pathogenic polarization of T H 17 cells through balanced type I IFN responses in human DCs. Article Snippet: Binding of IRF factors to the IFNB promoter was assessed by ChIP assays using the ChIP-IT Express Enzymatic Shearing and Article Title: Inhibiting KDM6A Demethylase Represses Long Non-Coding RNA Hotairm1 Transcription in MDSC During Sepsis. Article Snippet: The cells were transfected and incubated with RPMI-1640 medium for 36 h. Binding Assay:Article Title: YAP1-mediated regulation of mitochondrial dynamics in IDH1 mutant gliomas. Article Snippet: Jo u rn al o f Ce ll Sc ie n ce sheared enzymatically using Article Title: RORα is critical for mTORC1 activity in T cell-mediated colitis. Article Snippet: Antibodies anti-CD3e eBioscience Cat# 45-0031-82; RRID: AB_1107000 anti-CD4 Biolegend Cat# 100548; RRID: AB_2563054 anti-CD69 BD Biosciences Cat# 557392; RRID: AB_396675 anti-CD127 eBioscience Cat# 48-1271-82; RRID: AB_2016698 anti-Gr1 eBioscience Cat# 48-5931-82; RRID: AB_1548788 anti-CD11b eBioscience Cat# 47-0112-82; RRID: AB_1603193 anti-Siglec F eBioscience Cat# 46-1702-80; RRID: AB_2573724 anti-a4b7 Biolegend Cat# 120608; RRID: AB_10730607 anti-CCR9 eBioscience Cat# 11-1991-82; RRID: AB_657513 anti-CD45 eBioscience Cat# 11-0451-82; RRID: AB_465050 anti-CD45.1 BD Biosciences Cat# 553775; RRID: AB_10926208 anti-CD27 Biolegend Cat# 124216; RRID: AB_10639726 anti-RORgt BD Biosciences Cat# 562607; RRID: AB_11153137 anti-FOXP3 eBioscience Cat# 48-5773-82; RRID: AB_1518812 anti-Bcl-2 Biolegend Cat# 633507; RRID: AB_2043939 anti-IL-17A eBioscience Cat# 48-7177-82; RRID: AB_11149503 anti-IFN-g BD Biosciences Cat# 557998; RRID: AB_396979 anti-TNFa BD Biosciences Cat# 17-7321-82; RRID: AB_469508 anti-mTOR pS2448 eBioscience Cat# 50-9718-42; RRID: AB_2574352 anti-4EBP1 pT36/pT45 BD Biosciences Cat# 560285; RRID: AB_1645498 anti-S6 pS235/pS236 BD Biosciences Cat# 25-9007-42; RRID: AB_2637099 anti-AKT pS473 BD Biosciences Cat# 560397; RRID: AB_1645553 anti-AKT pT308 BD Biosciences Cat# 558275; RRID: AB_2225329 anti-RORa ThermoFisher Cat# PA5-11224; RRID: AB_2285213 Donkey anti-rabbit IgG Biolegend Cat# 406406; RRID: AB_1575135 anti-CD4 (IF) Biolegend Cat# 100443; RRID: AB_2562557 anti-CD127 (IF) BD Biosciences Cat# 552543; RRID: AB_394417 anti-CD69 (IF) BD Biosciences Cat# 557392; RRID: AB_396675 anti-HA CST Cat# 3724 Normal Rabbit IgG CST Cat# 2729 Chemicals, peptides, and recombinant proteins Recombinant Human TGF-b R&D Systems Cat# 240-B-010 Recombinant Mouse IL-6 Peprotech Cat# 216-16 Recombinant Mouse IL-23 R&D Systems Cat# 1887-ML-010 Recombinant Mouse IL-1b Peprotech Cat# 211-11B Critical commercial assays Fixation/Permeabilization Solution Kit BD Biosciences Cat# 554714 FOXP3 / Transcription Factor Staining Buffer Set eBioscience Cat# 00-5523-00 ChIP-IT Express Enzymatic Shearing Kit NEXTflex Active Motif Cat# 53035 (Continued on next page) Cell Reports 36, 109682, September 14, 2021 e1 Article Title: The U1 antisense morpholino oligonucleotide (AMO) disrupts U1 snRNP structure to promote intronic PCPA modification of pre-mRNAs Article Snippet: ChIP-Seq libraries were prepared using the Article Title: Reduced YAP1 and FOLR1 in gliomas predict better response to chemotherapeutics. Article Snippet: Gliomas harbouring mutations in IDH1 (isocitrate dehydrogenase 1) are characterized by greater sensitivity to chemotherapeutics.. These mutants also exhibit diminished levels of transcriptional coactivator YAP1 (yesassociated protein 1).. Enhanced DNA damage in IDH1 mutant cells, as evidenced by γH2AX formation (phosphorylation of histone variant H2A.X) and ATM (serine/threonine kinase; ataxia telangiectasia mutated) phosphorylation, was accompanied by reduced FOLR1 (folate receptor 1) expression. Article Title: Nuclear S100A9 Protein Induces Anti-Inflammatory Gene Expression in Sepsis Article Snippet: Article Title: FAM120A couples SREBP-dependent transcription and splicing of lipogenesis enzymes downstream of mTORC1. Article Snippet: In brief Cho et al. report FAM120A as a transcription co-activator that connects transcription and splicing of de novo lipid synthesis enzymes downstream of mTORC1.. Upon mTORC1 activation, SRPK2 phosphorylates splicing factor SRSF1, inducing its binding with FAM120A.. Through its interaction with SRSF1, the SREBP1-associated FAM120A promotes efficient splicing of lipogenic genes and facilitates lipid metabolism of cancer cells. Article Title: Fungal sensing by dectin-1 directs the non-pathogenic polarization of T H 17 cells through balanced type I IFN responses in human DCs. Article Snippet: Binding of IRF factors to the IFNB promoter was assessed by ChIP assays using the ChIP-IT Express Enzymatic Shearing and Article Title: Inhibiting KDM6A Demethylase Represses Long Non-Coding RNA Hotairm1 Transcription in MDSC During Sepsis. Article Snippet: The cells were transfected and incubated with RPMI-1640 medium for 36 h. Transfection:Article Title: YAP1-mediated regulation of mitochondrial dynamics in IDH1 mutant gliomas. Article Snippet: Jo u rn al o f Ce ll Sc ie n ce sheared enzymatically using Article Title: RORα is critical for mTORC1 activity in T cell-mediated colitis. Article Snippet: Antibodies anti-CD3e eBioscience Cat# 45-0031-82; RRID: AB_1107000 anti-CD4 Biolegend Cat# 100548; RRID: AB_2563054 anti-CD69 BD Biosciences Cat# 557392; RRID: AB_396675 anti-CD127 eBioscience Cat# 48-1271-82; RRID: AB_2016698 anti-Gr1 eBioscience Cat# 48-5931-82; RRID: AB_1548788 anti-CD11b eBioscience Cat# 47-0112-82; RRID: AB_1603193 anti-Siglec F eBioscience Cat# 46-1702-80; RRID: AB_2573724 anti-a4b7 Biolegend Cat# 120608; RRID: AB_10730607 anti-CCR9 eBioscience Cat# 11-1991-82; RRID: AB_657513 anti-CD45 eBioscience Cat# 11-0451-82; RRID: AB_465050 anti-CD45.1 BD Biosciences Cat# 553775; RRID: AB_10926208 anti-CD27 Biolegend Cat# 124216; RRID: AB_10639726 anti-RORgt BD Biosciences Cat# 562607; RRID: AB_11153137 anti-FOXP3 eBioscience Cat# 48-5773-82; RRID: AB_1518812 anti-Bcl-2 Biolegend Cat# 633507; RRID: AB_2043939 anti-IL-17A eBioscience Cat# 48-7177-82; RRID: AB_11149503 anti-IFN-g BD Biosciences Cat# 557998; RRID: AB_396979 anti-TNFa BD Biosciences Cat# 17-7321-82; RRID: AB_469508 anti-mTOR pS2448 eBioscience Cat# 50-9718-42; RRID: AB_2574352 anti-4EBP1 pT36/pT45 BD Biosciences Cat# 560285; RRID: AB_1645498 anti-S6 pS235/pS236 BD Biosciences Cat# 25-9007-42; RRID: AB_2637099 anti-AKT pS473 BD Biosciences Cat# 560397; RRID: AB_1645553 anti-AKT pT308 BD Biosciences Cat# 558275; RRID: AB_2225329 anti-RORa ThermoFisher Cat# PA5-11224; RRID: AB_2285213 Donkey anti-rabbit IgG Biolegend Cat# 406406; RRID: AB_1575135 anti-CD4 (IF) Biolegend Cat# 100443; RRID: AB_2562557 anti-CD127 (IF) BD Biosciences Cat# 552543; RRID: AB_394417 anti-CD69 (IF) BD Biosciences Cat# 557392; RRID: AB_396675 anti-HA CST Cat# 3724 Normal Rabbit IgG CST Cat# 2729 Chemicals, peptides, and recombinant proteins Recombinant Human TGF-b R&D Systems Cat# 240-B-010 Recombinant Mouse IL-6 Peprotech Cat# 216-16 Recombinant Mouse IL-23 R&D Systems Cat# 1887-ML-010 Recombinant Mouse IL-1b Peprotech Cat# 211-11B Critical commercial assays Fixation/Permeabilization Solution Kit BD Biosciences Cat# 554714 FOXP3 / Transcription Factor Staining Buffer Set eBioscience Cat# 00-5523-00 ChIP-IT Express Enzymatic Shearing Kit NEXTflex Active Motif Cat# 53035 (Continued on next page) Cell Reports 36, 109682, September 14, 2021 e1 Article Title: The U1 antisense morpholino oligonucleotide (AMO) disrupts U1 snRNP structure to promote intronic PCPA modification of pre-mRNAs Article Snippet: ChIP-Seq libraries were prepared using the Article Title: Reduced YAP1 and FOLR1 in gliomas predict better response to chemotherapeutics. Article Snippet: Gliomas harbouring mutations in IDH1 (isocitrate dehydrogenase 1) are characterized by greater sensitivity to chemotherapeutics.. These mutants also exhibit diminished levels of transcriptional coactivator YAP1 (yesassociated protein 1).. Enhanced DNA damage in IDH1 mutant cells, as evidenced by γH2AX formation (phosphorylation of histone variant H2A.X) and ATM (serine/threonine kinase; ataxia telangiectasia mutated) phosphorylation, was accompanied by reduced FOLR1 (folate receptor 1) expression. Article Title: Nuclear S100A9 Protein Induces Anti-Inflammatory Gene Expression in Sepsis Article Snippet: Article Title: FAM120A couples SREBP-dependent transcription and splicing of lipogenesis enzymes downstream of mTORC1. Article Snippet: In brief Cho et al. report FAM120A as a transcription co-activator that connects transcription and splicing of de novo lipid synthesis enzymes downstream of mTORC1.. Upon mTORC1 activation, SRPK2 phosphorylates splicing factor SRSF1, inducing its binding with FAM120A.. Through its interaction with SRSF1, the SREBP1-associated FAM120A promotes efficient splicing of lipogenic genes and facilitates lipid metabolism of cancer cells. Article Title: Fungal sensing by dectin-1 directs the non-pathogenic polarization of T H 17 cells through balanced type I IFN responses in human DCs. Article Snippet: Binding of IRF factors to the IFNB promoter was assessed by ChIP assays using the ChIP-IT Express Enzymatic Shearing and Article Title: Inhibiting KDM6A Demethylase Represses Long Non-Coding RNA Hotairm1 Transcription in MDSC During Sepsis. Article Snippet: The cells were transfected and incubated with RPMI-1640 medium for 36 h. Incubation:Article Title: YAP1-mediated regulation of mitochondrial dynamics in IDH1 mutant gliomas. Article Snippet: Jo u rn al o f Ce ll Sc ie n ce sheared enzymatically using Article Title: RORα is critical for mTORC1 activity in T cell-mediated colitis. Article Snippet: Antibodies anti-CD3e eBioscience Cat# 45-0031-82; RRID: AB_1107000 anti-CD4 Biolegend Cat# 100548; RRID: AB_2563054 anti-CD69 BD Biosciences Cat# 557392; RRID: AB_396675 anti-CD127 eBioscience Cat# 48-1271-82; RRID: AB_2016698 anti-Gr1 eBioscience Cat# 48-5931-82; RRID: AB_1548788 anti-CD11b eBioscience Cat# 47-0112-82; RRID: AB_1603193 anti-Siglec F eBioscience Cat# 46-1702-80; RRID: AB_2573724 anti-a4b7 Biolegend Cat# 120608; RRID: AB_10730607 anti-CCR9 eBioscience Cat# 11-1991-82; RRID: AB_657513 anti-CD45 eBioscience Cat# 11-0451-82; RRID: AB_465050 anti-CD45.1 BD Biosciences Cat# 553775; RRID: AB_10926208 anti-CD27 Biolegend Cat# 124216; RRID: AB_10639726 anti-RORgt BD Biosciences Cat# 562607; RRID: AB_11153137 anti-FOXP3 eBioscience Cat# 48-5773-82; RRID: AB_1518812 anti-Bcl-2 Biolegend Cat# 633507; RRID: AB_2043939 anti-IL-17A eBioscience Cat# 48-7177-82; RRID: AB_11149503 anti-IFN-g BD Biosciences Cat# 557998; RRID: AB_396979 anti-TNFa BD Biosciences Cat# 17-7321-82; RRID: AB_469508 anti-mTOR pS2448 eBioscience Cat# 50-9718-42; RRID: AB_2574352 anti-4EBP1 pT36/pT45 BD Biosciences Cat# 560285; RRID: AB_1645498 anti-S6 pS235/pS236 BD Biosciences Cat# 25-9007-42; RRID: AB_2637099 anti-AKT pS473 BD Biosciences Cat# 560397; RRID: AB_1645553 anti-AKT pT308 BD Biosciences Cat# 558275; RRID: AB_2225329 anti-RORa ThermoFisher Cat# PA5-11224; RRID: AB_2285213 Donkey anti-rabbit IgG Biolegend Cat# 406406; RRID: AB_1575135 anti-CD4 (IF) Biolegend Cat# 100443; RRID: AB_2562557 anti-CD127 (IF) BD Biosciences Cat# 552543; RRID: AB_394417 anti-CD69 (IF) BD Biosciences Cat# 557392; RRID: AB_396675 anti-HA CST Cat# 3724 Normal Rabbit IgG CST Cat# 2729 Chemicals, peptides, and recombinant proteins Recombinant Human TGF-b R&D Systems Cat# 240-B-010 Recombinant Mouse IL-6 Peprotech Cat# 216-16 Recombinant Mouse IL-23 R&D Systems Cat# 1887-ML-010 Recombinant Mouse IL-1b Peprotech Cat# 211-11B Critical commercial assays Fixation/Permeabilization Solution Kit BD Biosciences Cat# 554714 FOXP3 / Transcription Factor Staining Buffer Set eBioscience Cat# 00-5523-00 ChIP-IT Express Enzymatic Shearing Kit NEXTflex Active Motif Cat# 53035 (Continued on next page) Cell Reports 36, 109682, September 14, 2021 e1 Article Title: The U1 antisense morpholino oligonucleotide (AMO) disrupts U1 snRNP structure to promote intronic PCPA modification of pre-mRNAs Article Snippet: ChIP-Seq libraries were prepared using the Article Title: Reduced YAP1 and FOLR1 in gliomas predict better response to chemotherapeutics. Article Snippet: Gliomas harbouring mutations in IDH1 (isocitrate dehydrogenase 1) are characterized by greater sensitivity to chemotherapeutics.. These mutants also exhibit diminished levels of transcriptional coactivator YAP1 (yesassociated protein 1).. Enhanced DNA damage in IDH1 mutant cells, as evidenced by γH2AX formation (phosphorylation of histone variant H2A.X) and ATM (serine/threonine kinase; ataxia telangiectasia mutated) phosphorylation, was accompanied by reduced FOLR1 (folate receptor 1) expression. Article Title: Nuclear S100A9 Protein Induces Anti-Inflammatory Gene Expression in Sepsis Article Snippet: Article Title: FAM120A couples SREBP-dependent transcription and splicing of lipogenesis enzymes downstream of mTORC1. Article Snippet: In brief Cho et al. report FAM120A as a transcription co-activator that connects transcription and splicing of de novo lipid synthesis enzymes downstream of mTORC1.. Upon mTORC1 activation, SRPK2 phosphorylates splicing factor SRSF1, inducing its binding with FAM120A.. Through its interaction with SRSF1, the SREBP1-associated FAM120A promotes efficient splicing of lipogenic genes and facilitates lipid metabolism of cancer cells. Article Title: Fungal sensing by dectin-1 directs the non-pathogenic polarization of T H 17 cells through balanced type I IFN responses in human DCs. Article Snippet: Binding of IRF factors to the IFNB promoter was assessed by ChIP assays using the ChIP-IT Express Enzymatic Shearing and Article Title: Inhibiting KDM6A Demethylase Represses Long Non-Coding RNA Hotairm1 Transcription in MDSC During Sepsis. Article Snippet: The cells were transfected and incubated with RPMI-1640 medium for 36 h. Recombinant:Article Title: YAP1-mediated regulation of mitochondrial dynamics in IDH1 mutant gliomas. Article Snippet: Jo u rn al o f Ce ll Sc ie n ce sheared enzymatically using Article Title: RORα is critical for mTORC1 activity in T cell-mediated colitis. Article Snippet: Antibodies anti-CD3e eBioscience Cat# 45-0031-82; RRID: AB_1107000 anti-CD4 Biolegend Cat# 100548; RRID: AB_2563054 anti-CD69 BD Biosciences Cat# 557392; RRID: AB_396675 anti-CD127 eBioscience Cat# 48-1271-82; RRID: AB_2016698 anti-Gr1 eBioscience Cat# 48-5931-82; RRID: AB_1548788 anti-CD11b eBioscience Cat# 47-0112-82; RRID: AB_1603193 anti-Siglec F eBioscience Cat# 46-1702-80; RRID: AB_2573724 anti-a4b7 Biolegend Cat# 120608; RRID: AB_10730607 anti-CCR9 eBioscience Cat# 11-1991-82; RRID: AB_657513 anti-CD45 eBioscience Cat# 11-0451-82; RRID: AB_465050 anti-CD45.1 BD Biosciences Cat# 553775; RRID: AB_10926208 anti-CD27 Biolegend Cat# 124216; RRID: AB_10639726 anti-RORgt BD Biosciences Cat# 562607; RRID: AB_11153137 anti-FOXP3 eBioscience Cat# 48-5773-82; RRID: AB_1518812 anti-Bcl-2 Biolegend Cat# 633507; RRID: AB_2043939 anti-IL-17A eBioscience Cat# 48-7177-82; RRID: AB_11149503 anti-IFN-g BD Biosciences Cat# 557998; RRID: AB_396979 anti-TNFa BD Biosciences Cat# 17-7321-82; RRID: AB_469508 anti-mTOR pS2448 eBioscience Cat# 50-9718-42; RRID: AB_2574352 anti-4EBP1 pT36/pT45 BD Biosciences Cat# 560285; RRID: AB_1645498 anti-S6 pS235/pS236 BD Biosciences Cat# 25-9007-42; RRID: AB_2637099 anti-AKT pS473 BD Biosciences Cat# 560397; RRID: AB_1645553 anti-AKT pT308 BD Biosciences Cat# 558275; RRID: AB_2225329 anti-RORa ThermoFisher Cat# PA5-11224; RRID: AB_2285213 Donkey anti-rabbit IgG Biolegend Cat# 406406; RRID: AB_1575135 anti-CD4 (IF) Biolegend Cat# 100443; RRID: AB_2562557 anti-CD127 (IF) BD Biosciences Cat# 552543; RRID: AB_394417 anti-CD69 (IF) BD Biosciences Cat# 557392; RRID: AB_396675 anti-HA CST Cat# 3724 Normal Rabbit IgG CST Cat# 2729 Chemicals, peptides, and recombinant proteins Recombinant Human TGF-b R&D Systems Cat# 240-B-010 Recombinant Mouse IL-6 Peprotech Cat# 216-16 Recombinant Mouse IL-23 R&D Systems Cat# 1887-ML-010 Recombinant Mouse IL-1b Peprotech Cat# 211-11B Critical commercial assays Fixation/Permeabilization Solution Kit BD Biosciences Cat# 554714 FOXP3 / Transcription Factor Staining Buffer Set eBioscience Cat# 00-5523-00 ChIP-IT Express Enzymatic Shearing Kit NEXTflex Active Motif Cat# 53035 (Continued on next page) Cell Reports 36, 109682, September 14, 2021 e1 Article Title: The U1 antisense morpholino oligonucleotide (AMO) disrupts U1 snRNP structure to promote intronic PCPA modification of pre-mRNAs Article Snippet: ChIP-Seq libraries were prepared using the Article Title: Reduced YAP1 and FOLR1 in gliomas predict better response to chemotherapeutics. Article Snippet: Gliomas harbouring mutations in IDH1 (isocitrate dehydrogenase 1) are characterized by greater sensitivity to chemotherapeutics.. These mutants also exhibit diminished levels of transcriptional coactivator YAP1 (yesassociated protein 1).. Enhanced DNA damage in IDH1 mutant cells, as evidenced by γH2AX formation (phosphorylation of histone variant H2A.X) and ATM (serine/threonine kinase; ataxia telangiectasia mutated) phosphorylation, was accompanied by reduced FOLR1 (folate receptor 1) expression. Article Title: Nuclear S100A9 Protein Induces Anti-Inflammatory Gene Expression in Sepsis Article Snippet: Article Title: FAM120A couples SREBP-dependent transcription and splicing of lipogenesis enzymes downstream of mTORC1. Article Snippet: In brief Cho et al. report FAM120A as a transcription co-activator that connects transcription and splicing of de novo lipid synthesis enzymes downstream of mTORC1.. Upon mTORC1 activation, SRPK2 phosphorylates splicing factor SRSF1, inducing its binding with FAM120A.. Through its interaction with SRSF1, the SREBP1-associated FAM120A promotes efficient splicing of lipogenic genes and facilitates lipid metabolism of cancer cells. Article Title: Fungal sensing by dectin-1 directs the non-pathogenic polarization of T H 17 cells through balanced type I IFN responses in human DCs. Article Snippet: Binding of IRF factors to the IFNB promoter was assessed by ChIP assays using the ChIP-IT Express Enzymatic Shearing and Article Title: Inhibiting KDM6A Demethylase Represses Long Non-Coding RNA Hotairm1 Transcription in MDSC During Sepsis. Article Snippet: The cells were transfected and incubated with RPMI-1640 medium for 36 h. Staining:Article Title: YAP1-mediated regulation of mitochondrial dynamics in IDH1 mutant gliomas. Article Snippet: Jo u rn al o f Ce ll Sc ie n ce sheared enzymatically using Article Title: RORα is critical for mTORC1 activity in T cell-mediated colitis. Article Snippet: Antibodies anti-CD3e eBioscience Cat# 45-0031-82; RRID: AB_1107000 anti-CD4 Biolegend Cat# 100548; RRID: AB_2563054 anti-CD69 BD Biosciences Cat# 557392; RRID: AB_396675 anti-CD127 eBioscience Cat# 48-1271-82; RRID: AB_2016698 anti-Gr1 eBioscience Cat# 48-5931-82; RRID: AB_1548788 anti-CD11b eBioscience Cat# 47-0112-82; RRID: AB_1603193 anti-Siglec F eBioscience Cat# 46-1702-80; RRID: AB_2573724 anti-a4b7 Biolegend Cat# 120608; RRID: AB_10730607 anti-CCR9 eBioscience Cat# 11-1991-82; RRID: AB_657513 anti-CD45 eBioscience Cat# 11-0451-82; RRID: AB_465050 anti-CD45.1 BD Biosciences Cat# 553775; RRID: AB_10926208 anti-CD27 Biolegend Cat# 124216; RRID: AB_10639726 anti-RORgt BD Biosciences Cat# 562607; RRID: AB_11153137 anti-FOXP3 eBioscience Cat# 48-5773-82; RRID: AB_1518812 anti-Bcl-2 Biolegend Cat# 633507; RRID: AB_2043939 anti-IL-17A eBioscience Cat# 48-7177-82; RRID: AB_11149503 anti-IFN-g BD Biosciences Cat# 557998; RRID: AB_396979 anti-TNFa BD Biosciences Cat# 17-7321-82; RRID: AB_469508 anti-mTOR pS2448 eBioscience Cat# 50-9718-42; RRID: AB_2574352 anti-4EBP1 pT36/pT45 BD Biosciences Cat# 560285; RRID: AB_1645498 anti-S6 pS235/pS236 BD Biosciences Cat# 25-9007-42; RRID: AB_2637099 anti-AKT pS473 BD Biosciences Cat# 560397; RRID: AB_1645553 anti-AKT pT308 BD Biosciences Cat# 558275; RRID: AB_2225329 anti-RORa ThermoFisher Cat# PA5-11224; RRID: AB_2285213 Donkey anti-rabbit IgG Biolegend Cat# 406406; RRID: AB_1575135 anti-CD4 (IF) Biolegend Cat# 100443; RRID: AB_2562557 anti-CD127 (IF) BD Biosciences Cat# 552543; RRID: AB_394417 anti-CD69 (IF) BD Biosciences Cat# 557392; RRID: AB_396675 anti-HA CST Cat# 3724 Normal Rabbit IgG CST Cat# 2729 Chemicals, peptides, and recombinant proteins Recombinant Human TGF-b R&D Systems Cat# 240-B-010 Recombinant Mouse IL-6 Peprotech Cat# 216-16 Recombinant Mouse IL-23 R&D Systems Cat# 1887-ML-010 Recombinant Mouse IL-1b Peprotech Cat# 211-11B Critical commercial assays Fixation/Permeabilization Solution Kit BD Biosciences Cat# 554714 FOXP3 / Transcription Factor Staining Buffer Set eBioscience Cat# 00-5523-00 ChIP-IT Express Enzymatic Shearing Kit NEXTflex Active Motif Cat# 53035 (Continued on next page) Cell Reports 36, 109682, September 14, 2021 e1 Article Title: The U1 antisense morpholino oligonucleotide (AMO) disrupts U1 snRNP structure to promote intronic PCPA modification of pre-mRNAs Article Snippet: ChIP-Seq libraries were prepared using the Article Title: Reduced YAP1 and FOLR1 in gliomas predict better response to chemotherapeutics. Article Snippet: Gliomas harbouring mutations in IDH1 (isocitrate dehydrogenase 1) are characterized by greater sensitivity to chemotherapeutics.. These mutants also exhibit diminished levels of transcriptional coactivator YAP1 (yesassociated protein 1).. Enhanced DNA damage in IDH1 mutant cells, as evidenced by γH2AX formation (phosphorylation of histone variant H2A.X) and ATM (serine/threonine kinase; ataxia telangiectasia mutated) phosphorylation, was accompanied by reduced FOLR1 (folate receptor 1) expression. Article Title: Nuclear S100A9 Protein Induces Anti-Inflammatory Gene Expression in Sepsis Article Snippet: Article Title: FAM120A couples SREBP-dependent transcription and splicing of lipogenesis enzymes downstream of mTORC1. Article Snippet: In brief Cho et al. report FAM120A as a transcription co-activator that connects transcription and splicing of de novo lipid synthesis enzymes downstream of mTORC1.. Upon mTORC1 activation, SRPK2 phosphorylates splicing factor SRSF1, inducing its binding with FAM120A.. Through its interaction with SRSF1, the SREBP1-associated FAM120A promotes efficient splicing of lipogenic genes and facilitates lipid metabolism of cancer cells. Article Title: Fungal sensing by dectin-1 directs the non-pathogenic polarization of T H 17 cells through balanced type I IFN responses in human DCs. Article Snippet: Binding of IRF factors to the IFNB promoter was assessed by ChIP assays using the ChIP-IT Express Enzymatic Shearing and Article Title: Inhibiting KDM6A Demethylase Represses Long Non-Coding RNA Hotairm1 Transcription in MDSC During Sepsis. Article Snippet: The cells were transfected and incubated with RPMI-1640 medium for 36 h. |